1, bacteria strain selection
Service on veterinarian microorganism, food microorganism and biological agriculture chemical bacterial selection are offered in this laboratory. The scheme is selectively put forward according to the requirements of the customers.

2, protein expression and purification in prokaryote

It has much value to introduce genes into E.coli, and express large amount of protein for protein purification, orientation and its function analyses.

The service offered as following:

The cloning of purpose gene: we have many prokaryote express carrier (for instance: PET System, QIAexpress System) for the customer to choose 。 it can totally meet different experiment requirements. We can offer and clone good plasmid, bacterial strain and check report in 2 to 3 weeks.

Prokaryote protein expression and purification: We divide the course into three as: the preliminary, experiment of the fermented condition and purification craft determitation in earlier stage; generous protein preparation and protein appraise. the time is 1 to 3 months. Finally we will offer you: ⑴Preliminary fermented parameters; ⑵ a rational, enlargeable Purification craft; ⑶the purified protein is about 10mg. the SDS-PAGE protein electrophoresis result is detailed offered.

The handed protein will be examined in order to confirm the exactness of its expression through Western. The Western antibody is offered by the customer.

3, the expression carrier construction of eukaryote and prokaryote
Gene and its expression carriers are offered by customers. The procedure is confirmed either by the customer or the library.

4 , expression and purification of eukaryotes
It is much valuable for Methods to use a large number of proteins expression in protein purification, function analyzed and clinical practice after the gene be cloned into Pichia yeast. There are more than 30 kinds of exogenous gene s successively expressed in the system, the majority of which is medical products such as Interleukin- 2, Human serum albumin,(HAS) , tumor necrosis factor, （ TNF) , hepatitis B virus-major surface antigen , ramification of Lupus anticoagulant factor Lepirudin etc., some of them will enter the market soon, and some have done. We can also express natural protein and molecule in Pichia yeast, to study its function. The expression and craft of the purification we offered are shown in entry 4 in technique.

5, preparation of antibody
The production content for entrusted antibody: chicken, rabbit, and polyclonal antibody.

The following articles are needed offering by the customer

(1) intended cDNA or EST cloning

(2) intended antigen (protein or polypeptide)

Serve achievement:

(1) High titer specific hetero-antibody, at least20mg.

(2) Offer the corresponding ELISA report;

(3) According to the needs of customer, we can offer Western Blot and immune group method, Including all experimental data and photos;